The nucleus reforms and the cell divides. Cohesin proteins break down and the sister chromatids separate. Location and Checks and Balances Kinetochores form in the central region, or centromere, of a duplicated chromosome. These fibers work together to separate chromosomes during cell division. Sister chromatids line up at the metaphase plate. In cell biology, the spindle apparatus is the cytoskeletal structure of eukaryotic cells that forms during cell division to separate sister chromatids. Kinetochore fibers extend from the kinetochore region and attach chromosomes to microtubule spindle polar fibers. The kinetochore becomes attached to the mitotic spindle.The nucleus reforms and the cell divides. Spindle fibers are cellular structures that serve a function during cell division processes. The kinetochore breaks down and the sister chromatids separate. Sister chromatids line up at the metaphase plate. The kinetochore becomes attached to the cohesin proteins.Centromere divides, producing two sister chromatids, now known as daughter chromosomes, with the spindle fibers attached to each chromosome. The centrioles are now at opposite poles in each of the daughter cells. Soon after, the cell divides in two, resulting in daughter cells with identical DNA. The nucleus reforms and the cell divides. Chromosomes arrange on the equator of the cell with the help of the spindle fibers. During cell division, spindle fibers attach to the centromere and pull each of the sister chromatids to opposite sides of the cell. ![]() The kinetochore becomes attached to the mitotic spindle. The microtubules start growing toward the opposite end of the cell.
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